Sterilization in dry oven:
- A- one hour at 160 c.
- B- 90 minutes at 160 c.
- C- two hours at 160 c.
- D- none.
Option C: Two hours at 160°C (320°F) is the recommended time and temperature for sterilization in a dry oven.
Here's why:
- Effectiveness:
Dry heat sterilization relies on the denaturing of proteins and enzymes in microorganisms to kill them. 160°C for two hours is sufficient to eliminate most bacteria, fungi, and even some spores, which are more resistant.
- Safety:
Higher temperatures or longer exposure times can damage heat-sensitive materials like some plastics or fabrics. Sticking to 160°C for two hours ensures effective sterilization while minimizing the risk of damaging your items.
- Standardized practice:
160°C for two hours is a common recommendation in laboratory and medical settings for sterilizing glassware, instruments, and other dry goods.
Additional Tips:
Here are some additional tips for dry oven sterilization:
- Pre-cleaning:
Make sure your items are clean and free of debris before placing them in the oven.
- Packaging:
Wrap items individually in heat-resistant materials like aluminum foil or sterilization pouches to prevent contamination after sterilization.
- Placement:
Arrange items in the oven so that they are not touching each other or the oven walls to ensure even heat distribution.
- Cool-down:
Allow the oven to cool down completely before removing items.
