Saturday, December 16, 2017

Nucleic Acid Cutters.. Specific number of nitrogen bases. Cut the DNA from donor cells and plasmid with the same restriction enzyme



In the cell there are types of enzymes that cut DNA at specific regions. These enzymes are known as the restriction of internal nucleic acids.
The cutting zones are characterized by a specific number of nitrogen bases.
These enzymes can cut DNA fissures in asymmetrical regions leading to single-wick limbs, and facilitate adhesion of these limbs to other complementary limbs.
In this case, the DNA of donor cells and plasmid is cut off with the same restriction enzyme for the development of end-ends between DNA and plasmid. When the pieces of DNA are added to the plasmid parts, a new plasmid carrying part of the harvested DNA can be inserted into the bacterial cell to multiply within it.
Other enzymes, known as binding enzymes, have been shown to close the vacuum created by the restriction enzyme. In this way, the plasmid recovers its annular shape.