Friday, April 19, 2024

Lamb in Flow cytometry.. Laser

Lamb in Flow cytometry:

  • A. Halogen.
  • B. Light.
  • C. Laser.
  • D. Electrons.

The correct answer is: C. Laser

Here's why:

- Halogen:

While halogen lamps can be used in some flow cytometers, they are not the main source of excitation light for analyzing cells. They are typically used for side scatter and forward scatter measurements.

- Light:

Broad-spectrum light is not specific enough to excite different fluorochromes used in flow cytometry. Lasers provide focused, monochromatic light that can efficiently excite specific fluorochromes, allowing for accurate identification of different cell populations.

- Electrons:

Electrons are not directly involved in the excitation process in flow cytometry. They are used in the detectors to measure the fluorescence emitted by the cells.

- Laser:

Lasers are the primary source of excitation light in most modern flow cytometers. They emit a narrow, intense beam of light that can be precisely tuned to the excitation wavelengths of specific fluorochromes. This allows for efficient excitation and discrimination of different cell populations based on their fluorescent markers.
Therefore, the correct answer is C. Laser.